Answer :
The movement that called into question the rule of kings and proposed that people had basic human rights, threatening the Spanish Empire in the Americas is: B. The Enlightenment.
The Enlightenment was an intellectual movement that held sway in Europe in the 17th and 18th centuries. This movement was marked by a rejection of religious intolerance and absolute monarchies.
It advocated for liberty, separation of the church and state, and a constitutional government. Reason preceded during this period.
This movement threatened the absolute power that the Spanish Empire held in the Americas.
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