Answer :
Unintended effects might a healthcare worker who is practicing poor handwashing techniques have on patient diagnosis The worker's normal flora might contaminate patient samples, leading to misdiagnosis. The results of the experiment might be compromised.
Flame the necks of bottles and test tubes
This will prevent microorganisms from entering the mouth of the vessel and contaminating the culture or medium. Passing the finish of the bottle through a flame creates convection through the finish and helps prevent contamination.
Framing before use kills bacteria on the loop that can contaminate the culture. Framing after use kills any bacteria left in the loop from bacterial migration activity.
Why is the mouth of the culture tube passed through a Bunsen burner flame before the cap is removed and replaced? To prevent the entry of outside air that can introduce contaminants.
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